open_UMR

Aaron Beller

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Willkommen auf open_UMR!

Open_UMR ist ein digitales Repositorium: Ein fachübergreifendes Publikationsportal, das wissenschaftliche Textpublikationen, Ressourcen, Forschungsdaten und Software von Universitätsangehörigen der Philipps-Universität sammelt, verfügbar hält und im open access offen zugänglich macht. Im Sinne einer hohen Qualität sowie einer potentiellen Nachnutzbarkeit ist dieses Repositorium kuratiert.

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Open Access
Point-of-Care Ultrasound Pulse Checks During Cardiopulmonary Resuscitation on a Patient Simulator (PUPRAS)
(MDPI) Betz, Susanne; Bergmann, Harald; Rettich, Franz; Kreutz, Julian; Ploeger, Birgit; Jaenig, Christoph W.; Grosch, Stephan; Meggiolaro, Karl M.; Jerrentrup, Andreas; Schmidbauer, Willi; Schieffer, Bernhard; Gruebl, Tobias
Background/Objectives: During cardiopulmonary resuscitation (CPR), patients must be checked for signs of return of spontaneous circulation (ROSC). Point-of-care ultrasound (POCUS) may be more reliable for detecting the ROSC. We investigated whether a POCUS pulse check algorithm could be used in compliance with the CPR guidelines. Methods: This was a prospective controlled and blinded multicentre manikin study involving staff from two tertiary clinical centres and their emergency medical services. A standard operating procedure for POCUS pulse checks during CPR was evaluated using a simulator in a team of four. The POCUS pulse checks were performed at the central artery following basic and advanced life support. The first pulse check was performed in the setting of pulseless electrical activity, and the second was performed in the presence of ROSC. The participants also completed a questionnaire. Results: A total of 444 pulse checks (244 manual/200 POCUS) were performed in 100 scenarios. The participants comprised physicians (34%), nurses (15%), non-physician emergency medical services personnel (37%), and other medical personnel (14%). The pulse checks took an average of 6.7 s (SD 3.9 s). Manual pulse checks (7.3 s) took longer than ultrasound pulse checks (6.1 s; p < 0.01), which were performed after a mean of 7.1 min (SD 1.7 min), during the fourth rhythm analysis in 93% of cases, and at the femoral artery in 62% of cases. They were rated as “easy” to perform by 77% and “useful” by 94%. Conclusions: POCUS pulse checks basically seem easy to implement and appear to be feasible during CPR.
Open Access
Validation of the metabolic power model during three intermittent running-based exercises with emphasis on aerobic and anaerobic energy supply
(Frontiers) Brochhagen, Joana; Hoppe, Matthias W.
Introduction: In intermittent sports, available internal load measurements like capillary blood techniques and portable respiratory gas analyzers are considered as gold standards in controlled laboratory environments, but are impractical for daily use in training and matches. A newer approach, the metabolic power model, allows to extrapolate from speed and acceleration data to the metabolic power, simulated oxygen uptake, and aerobic and anaerobic energy supply. The aim of this study was to validate the metabolic power model against the established 3-component model to allow direct comparison of variables including energy expenditure and supplies during intermittent running-based exercises. Methods: Twelve male athletes (24 ± 3 years) performed three different runningbased exercises consisting of continuous shuttle runs and repeated accelerations and sprints with change of direction. Each exercise condition intended to primarily stress the aerobic, anaerobic alactic, and lactic energy supply. One-way repeated measures ANOVA or Friedman test and corresponding effect sizes were applied for statistical analyses. Additionally, absolute and relative biases and Bland-Altman plots were generated. Results: For total energy expenditure, there were statistically significant differences (p ≤ .002, d ≥ .882, large) and biases of −13.5 ± 11.8% for the continuous shuttle runs and up to 352.2 ± 115.9% for repeated accelerations and sprints. Concerning aerobic energy supply, there were statistically significant differences (p < .001, d ≥ 1.937, large effect sizes) and biases of up to −38.1 ± 11.7%. For anaerobic energy supply, there were statistically significant differences (p < .001, d ≥ 5.465, large) and biases of up to 1,849.9 ± 831.8%. Discussion: In conclusion, the metabolic power model significantly under- or overestimates total energy expenditure and supplies with large effect sizes during intermittent running-based exercises. Future studies should optimize the model before it can be used on a daily basis for scientific and practical purposes.
Open Access
Heterozygous Men1(+/T) Knockout Mice Do Not Develop Bronchopulmonary Neuroendocrine Hyperplasia or Neoplasia but Bronchial Adenocarcinoma
(MDPI) Albers, Max Benjamin; Fink, Ludger; Manoharan, Jerena; Lopez, Caroline L.; Bollmann, Carmen; Bartsch, Detlef K.
Introduction: Bronchopulmonary Neuroendocrine Neoplasms (NEN) occur in 2–7% of patients with multiple endocrine neoplasia type 1 (MEN1). Precursor lesions have been identified for MEN1-related pancreatic, duodenal, and gastric NEN. The aim of the current study using a MEN1 mouse model was to define the precursor lesions of bronchopulmonary NEN and evaluate the potential prophylactic antitumor effects of somatostatin analogues in a transgenic MEN1 mouse model. Methods: Fifteen mice, germline heterozygous for Men1(+/T), were treated with subcutaneous injections of lanreotide autogel (Somatuline Autogel®, IPSEN Pharma), while 15 mice were treated with subcutaneous injections of physiologic sodium chloride as the control group. Five mice from each group were euthanized after 12, 15, and 18 months, respectively. The complete lungs were resected and evaluated after hematoxylin and eosin staining and immunohistochemistry for synaptophysin and chromogranin A. Results: In the lungs of the 30 evaluated mice, whether treated or placebo treated, no bronchopulmonary neuroendocrine cell hyperplasia nor neuroendocrine neoplasia was detected through histopathology. However, pulmonary adenocarcinoma developed in 2 (13%) of the 15 untreated mice and in 1 (7%) of the 15 lanreotide-treated mice. Conclusions: Heterozygous Men1(+/T) knockout mice do not develop bronchopulmonary NEN or precursor lesions, but pulmonary adenocarcinoma. This surprising result needs to be investigated in more detail.
Open Access
Immunopathological Dysregulation in Acute Myeloid Leukemia: The Impact of T-bet, RORγt, and FOXP3 on Disease Dynamics
(MDPI) Mohy El-Din, Amira M. Mohamed; AlShaarawy, Buthayna Ahmad; Kandeel, Eman Zaghloul; AlDewi, Dalia Mahmoud; Refaat, Lobna Abdel Azeem; Arneth, Borros; Sabit, Hussein
The etiology of acute myeloid leukemia (AML) is complex, including genetic and environmental abnormalities. The immune system anomalies play an essential role in the process of leukemogenesis. However, the immunopathological factors, including abnormal T helper (Th) subsets, contributing to the initiation and progression of this neoplasm, require further investigation. Considering the previously mentioned data, we decided to study the expression pattern of transcription factors T-bet, Foxp3, and RORγt that regulate Th1, Treg, and Th17, respectively, in acute myeloid leukemia with correlation to clinical and other investigation data and treatment outcomes. This study was conducted on 80 newly diagnosed patients with AML recruited from the National Cancer Institute, Cairo University, and 25 healthy control subjects. The AML patient cohort consisted of 30 females (37.5%) and 50 males (62.5%), ranging from 18 to 74 years old. The control group was 8 females (32%) and 17 males (68%), with ages ranging from 23 to 40 years old. Samples were provided from the bone marrow of donor cases for allogeneic bone marrow transplantation. The diagnosis of acute myeloid leukemia was based on morphologic and cytochemical evaluation, immunophenotyping, and complementary cytogenetics according to WHO criteria. Upshift from the normal T-bet intensity of power (MFI), RORγt+ CD4+ T lymphocyte frequency (%) with downshift from the normal FOXP3 intensity of power (MFI), may suggest a state of inflammation. In contrast, an upshift from the normal FOXP3+ CD4+ T lymphocyte frequency (%) may reflect a state of immunosuppression in the bone marrow microenvironment of AML. Combined, they constitute a sophisticated scenario of immunological disorder in AML. Co-expression of T-bet and RORγt transcription factors in CD4+ T lymphocytes in both normal and AML groups may suggest CD4+ T lymphocyte plasticity.
Open Access
Anti-Tumor Potential of Frankincense Essential Oil and Its Nano-Formulation in Breast Cancer: An In Vivo and In Vitro Study
(MDPI) Mohamed, Nouran; Ismail, Hisham; Nasr, Ghada M.; Abdel-Ghany, Shaimaa; Arneth, Borros; Sabit, Hussein
Background/Objective: Breast cancer remains the most common malignancy among women worldwide, contributing to high morbidity and mortality rates. Many anticancer drugs have been derived from medicinal plants, and frankincense from Boswellia carterii is notable for its anti-inflammatory, anti-neoplastic, and anti-carcinogenic properties. Using gas chromatography/mass spectrometry (GC/MS), 48 components were identified in B. carterii essential oil, and the major constituent was α-pinene (35.81%). Method: In this study, we investigated the anti-tumor effects of frankincense essential oil (FEO) and its nano-formulation with chitosan (FEO-CSNPs) using in vitro breast cancer models (MCF- 7, MDA-MB-231, and 4T1 cells) and in vivo mouse mammary carcinoma (4T1) models (Balb/c). Results: The results showed significant reductions in cell viability. At 10 μg/mL, the FEO showed the highest reduction in the C-166 cells, while at 100 μg/mL, the FEO exhibited a stronger cytotoxicity in the MDA-MB-231 and 4T1 cells compared to the FEOCSNPs and CSNPs. The FEO-CSNPs exhibited cell growth arrest in the S, G2/M, and G1/S phases in the MCF-7, MDA-MB-231, and 4T1 cell lines (36.91%, 23.12%, and 33.58%), in addition to increased apoptosis rates in the MCF-7, MDA-MB-231, and 4T1 cell lines (33.04%, 36.39%, and 42.19%). The wound healing assays revealed a decreased migratory ability in the treated cells. The in vivo experiments in the balb/c mice demonstrated a reduction in the tumor volume, with a histopathological analysis confirming extensive tumor necrosis. Moreover, the FEO and FEO-CSNPs showed notable antioxidant and arginase activity. The gene expression analysis via qPCR indicated the upregulation of tumor suppressor genes and the downregulation of oncogenes. Conclusions: These findings suggest that FEO and its nano-formulation, particularly in the form of FEO-CSNPs as an oral formulation, display enhanced efficacy, warranting further preclinical and clinical research to develop innovative treatment strategies.