Identifizierung und Charakterisierung des Mais-spezifischen Signalpeptids Zip1 und dessen Aktivierung durch Cysteinproteasen
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Philipps-Universität Marburg
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Abstract
During their development plants face a wide range of pathogens. Control of plant pathogen resistance or susceptibility depends on the lifestyle of the invader and the promotion of either cell survival or cell death. Papain-like cysteine proteases (PLCPs) play important roles in regulating plant defense responses and in protecting plants against biotrophic pathogens. In maize (Zea mays) a set of five PLCPs, namely CP1A, CP1B, CP2, XCP2 and CatB, is critical in the orchestration of salicylic acid (SA)-dependent signaling. Intriguingly, the interaction of PLCPs and SA signaling pathways are non-linear and display patterns of reciprocal amplification. Underlying biochemical mechanisms by which PLCPs promote SA-associated defense responses remain unknown. In this work a peptide in apoplastic fluid of SA treated leaves termed Zip1 (Zea mays immune signaling peptide 1) was identified by using mass spectrometry and maize leaf bioassays. Zip1 induces maize PR-gene expression and activates apoplastic PLCPs similar to SA. In vitro studies using recombinant proteins demonstrate that CP1 and CP2 release immunogenic peptide signals from the propeptide precursor PROZIP1. Furthermore, RNAseq-based transcriptome analyses in maize leaves revealed that Zip1 treatment almost completely resembles SA defense responses. In line with that, Zip1 treatment results in de novo synthesis of SA. In the context of a necrotrophic fungal pathogen (Botrytis cinerea), Zip1 promotes disease susceptibility towards maize.
Zip1, an endogenous plant peptide released by PLCPs, is the first immunogenic peptide, which acts sequentially as an amplifier of SA-dependent defense signaling in maize.
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Created: 2017Issued: 2017-10-04Updated: 2017-10-09
Faculty
Fachbereich Biologie
Publisher
Philipps-Universität Marburg
Language
ger
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DoctoralThesis
DFG-subjects
Pflanzen ImmunitätSignalpeptidPapain-ähnliche Cysteinproteasen
DDC-Numbers
570
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Ziemann, Sebastian: Identifizierung und Charakterisierung des Mais-spezifischen Signalpeptids Zip1 und dessen Aktivierung durch Cysteinproteasen. : Philipps-Universität Marburg 2017-10-04. DOI: https://doi.org/10.17192/z2017.0668.