In this thesis, BGCs for secondary metabolites from A. ustus have been investigated via multiple approaches. The identified PKS-containing utt BGC and two similar NRPS-containing opa and opa2 BGCs from the same fungus A. ustus demonstrated clearly the large fungal potential for natural product production and new enzyme discovery. In addition, site-directed mutagenesis for a fungal PT led to the functional switching with enhanced conversion yields, indicating that site-directed mutagenesis is an effective tool to increase the structural diversity of natural products.
Zheng, Liujuan: Investigations on the biosynthesis of secondary metabolites and biosynthetic enzymes from Aspergillus species. : Philipps-Universität Marburg 2021-06-08. DOI: https://doi.org/10.17192/z2021.0219.