Item type:Thesis, Open Access

Durchflusszytometrische Untersuchung zur Frage der Absorption und Elution von HLA-Klasse I-Antigenen auf Thrombozyten während der Lagerung als gepoolte Thrombozytenkonzentrate

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Date

2006-10-20

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Philipps-Universität Marburg
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Abstract

The origin of HLA class I molecules on platelets is still under discussion. Adsorption of HLA molecules on platelets using specific experimental conditions has been described. The study presented investigates whether there is a significant elution and adsorption of HLA class I molecules on platelets during storage of pooled random platelet concentrates (PRPC) under routine conditions. Platelet concentrates (PCs) from whole blood prepared from HLA-A2-positve and HLA-A2-negative donors, pooled and stored under routine conditions. In addition, platelets from HLA-A2-negative donors were pelleted and resuspended in cell-free plasma from HLA-A2-positiv donors. HLA-A2-positive PCs (positive control), HLA-A2-negative PCs (negative control) and HLA-A2-negative platelets in plasma from HLA-A2-negative donors were stored simultaneously. Binding of FITC-conjugated monoclonal murine antihuman Anti-HLA2 antibodies (anti-HLA-A2-mab) was measured during 5-day storage by flow cytometry. An increased binding of anti-HLA-A2-mab during storage was found on HLA-A2-negative platelets (p<0,005) independently whether they were incubated with cell-free plasma or platelets from HLA-A2-positve donors or autologous HLA-A-negative cell-free plasma. However, non-specific binding of IgG controls increased equally, whereas anti-HLA-A2-mab binding to platelets from HLA-A2-positive donors did not decrease during storage. The study suggests that there is no significant elution and adsorption of HLA class I antigens of platelets in pooled PCs during storage under the usual conditions for platelet storage. Increased anti-HLA-A2-mab signal was due to non-specific binding. Therefore, HLA class I compatible platelets should maintain their compatibility for an immunized patient when stored in a pool with HLA incompatible platelets and shortened survival after transfusion should not be expected.

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Dates
Created: 2006Issued: 2006-10-20Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2006.0719
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
PlateletGepoolte ThrombozytenkonzentrateHLA-AntigenHLA-antigenThrombozytentransfusionPlatelet transfusionPlatelet storage
DFG-subjects
Thrombozyt
DDC-Numbers
610
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2006.0719
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Heim, Markus (132195321): Durchflusszytometrische Untersuchung zur Frage der Absorption und Elution von HLA-Klasse I-Antigenen auf Thrombozyten während der Lagerung als gepoolte Thrombozytenkonzentrate. : Philipps-Universität Marburg 2006-10-20. DOI: https://doi.org/10.17192/z2006.0719.

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