Item type:Thesis, Open Access

Experimentelle Ansätze zur Untersuchung spezifischer Prozessierung extrazytoplasmatischer Proteine durch drei allele Signalpeptidasen aus Bradyrhizobium japonicum

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Date

2009-03-25

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Philipps-Universität Marburg
DOI

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Abstract

Several gene disruption mutants of B. japonicum showing symbiotic phenotypes arose from recombination experiments using a bank of gene-marker-fusions. Using three different experimental approaches the significance of genes coding for extracytoplasmic proteins in establishing a functional symbiosis with soy bean (G. max) was examined. Specific DNA amplification was used to verify the correct manipulation of the disrupted gene regions. Second, a strategy was developed to mutagenise the nex18 gene region. This gene region exists twice in the B. japonicum genome showing absolute sequenc homology, thus procedures previously used to mutagenise other gene regions were uneffective. To test for a substrate specificity of the three signal peptidases of B. japonicum, a third project was initiated to replace the lepB gene of E. coli by one of the B. japonicum signal peptidase genes (sipF, sipS, sipX).

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Supervisor:
Dates
Created: 2009Issued: 2009-03-25Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2009.0100
Faculty
Fachbereich Biologie
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
SignalpeptidaseProteinExtracytoplasmicProteinExtrazytoplasmatischProzessierungSpezifischSignal peptidaseSpecificProcessing
DFG-subjects
BradyrhizobiumRhizobium japonicum
DDC-Numbers
570
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2009.0100
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Nilles, Patrick (137679440): Experimentelle Ansätze zur Untersuchung spezifischer Prozessierung extrazytoplasmatischer Proteine durch drei allele Signalpeptidasen aus Bradyrhizobium japonicum. : Philipps-Universität Marburg 2009-03-25. DOI: https://doi.org/10.17192/z2009.0100.

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This item has been published with the following license: In Copyright