Item type:Thesis, Open Access

Localization and identification ofneuropeptides in antennal lobe neurons of thesphinx moth Manduca sexta

Thumbnail Image

Files

dsu.pdf (77.87 MB)

Date

2007-10-29

Authors

Publisher

Philipps-Universität Marburg
DOI

Supervisors

Abstract

Indispensable prerequisites to understanding the functional role of neuropeptides during CNS ontogeny are (1) their identity (neuropeptidome), (2) their temporal and (3) spatial occurrence. Based on these findings, expression patterns of identified neuropeptides can be correlated with defined events during development. Corresponding experiments shed light on the function and regulation of these molecules. In the present work, the appearance of neuropeptides during development was studied in the antennal lobes (ALs) of the sphinx moth Manduca sexta. The following results were obtained: (1) Using MALDI-TOF mass spectrometry, ion signal profiles of lateral cell groups from the ALs were recorded during development. These profiles revealed the presence of twelve chemically identified neuropeptides, as well as the patterns of their temporal occurrence (UTZ et al. 2007a). (2) Immunocytochemical staining was used to detect peptides of the A-type allatostatin family (AST-A) and Manduca sexta allatotropin (Mas-AT) in cells of the developing AL and allowed a detailed analysis of their cellular localization and temporal appearance (UTZ and SCHACHTNER 2005; UTZ et al. 2007b). (3) Manipulation of the hemolymph titer of 20-hydroxyecdysone (20E) showed that peptide expression in the different neuron types of the AL is controlled by levels of 20E during development (UTZ and SCHACHTNER 2005; UTZ et al. 2007b).

Review

Metadata

Contributors
Supervisor:
Dates
Created: 2007Issued: 2007-10-29Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2007.0517
Faculty
Fachbereich Biologie
Publisher
Philipps-Universität Marburg
Language
eng
Data types
DoctoralThesis
Keywords
Nervous systemNeuropeptidesInvertebratesCNSOlfactory systemInsectsAntennal lobe
DFG-subjects
Insekten, Olfaktorisches SystemNervensystemInvertebratenNeuropeptideAntennallobusZNS
DDC-Numbers
590
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2007.0517
show more
Utz, Sandra: Localization and identification ofneuropeptides in antennal lobe neurons of thesphinx moth Manduca sexta. : Philipps-Universität Marburg 2007-10-29. DOI: https://doi.org/10.17192/z2007.0517.

License

item.page.license.under-copyright
This item has been published with the following license: In Copyright