Item type:Thesis, Open Access

Identifizierung neuer pro-migratorischer Gene in der angeborenen Immunabwehr unter Verwendung eines Drosophila Makrophagen in vivo Zellkultursystems

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2026-04-20

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The ability to migrate is important for many cellular processes that shape cells into a multicellular organism and later enable adaptation to changing environmental conditions. The force-generating motor is the actin cytoskeleton, whose organization and dynamics play a fundamental role in building cell shape and enabling migration. The fruit fly Drosophila melanogaster has a blood cell system that functions similar like the hematopoietic system of vertebrates. In this study we investigated plasmatocytes, which are special cells of the immune system that are comparable to mammalian macrophages. Metamorphosis requires an effective system to break down the larval tissue structures and requires cells that can migrate throughout the body. Lehne et al. identified a variety of genes that are upregulated during the transition from the larval to the pupal stage. We used the resulting list of genes with varying expression levels for a systematic RNAi screening to identify genes whose products influence cell migration. The vrp1 gene, whose translation product belongs to the verproline family, showed reduced lamellipodia and increased filopodia formation after RNAi-mediated suppression. Quantification revealed a significant reduction in cell circularity and roundness of vrp1-depleted cells. Subsequent analysis of motility showed that the vrp1-depleted cells moved significantly more slowly, suggesting a migration defect. We also found a reduced WASP concentration in the knock-down macrophages. Future studies have to reveal how Vrp1/WIP acts through WASP to regulate cells shape and motility. The second gene we examined was the heat shock protein Hsp83, which stood out after expression-suppression with small cells and irregular lamellipodia formation. Quantification revealed a significantly reduced cell size after both lectin-mediated and integrin-mediated proliferation. Cell circularity and roundness were also significantly reduced. The evaluation of the live cell microscopy showed a relevant reduction in the average cell speed and the average distance covered. We could not determine any effect on the WAVE or WASP concentration of hsp83-RNAi expressing cells.

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Issued: 2026-04-20
DOI
https://doi.org/10.17192/openumr/696
Faculty
FB20:Medizin
Language
de
Keywords
DrosophilaMigrationAktinZytoskelettMakrophagen
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Attribution 4.0 International
URI
https://open.uni-marburg.de/handle/openumr/10437
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Murken, Catharina: Identifizierung neuer pro-migratorischer Gene in der angeborenen Immunabwehr unter Verwendung eines Drosophila Makrophagen in vivo Zellkultursystems. : 2026-04-20. DOI: https://doi.org/10.17192/openumr/696.

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Attribution 4.0 International
Except where otherwised noted, this item's license is described as Attribution 4.0 International

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