Item type:Thesis, Open Access

Induktion von Apoptose durch ungesaettigte Fettsaeuren in Zellen des kardiovaskulaeren Systems

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Date

2007-06-08

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Philipps-Universität Marburg
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Abstract

Unsatturated fatty acids like oleic acid are shown to activate the protein phosphatase type 2C (PP2C) in vitro and induce apoptosis in cultivated human endothelial cells. The main goal of this work was to analyze whether unsatturated fatty acids from human lipoproteins could induce apoptosis in cultivated endothelial cells (HUVECs). Therefore HUVECs were co-cultivated with THP-1 macrophages to establish a new cell culture model which simulates the mechanism of fatty acid release in the vascular wall. Co-cultivated HUVECs were apoptoticaly damaged after addition of human VLDL in physiological range. Furthermore, the consequences of a oleic acid-rich diet was investigated on guinea pigs. The animals received a diet enriched with 25% Glyceryl Trioleate for 5 months. Verum animals (25% Trioleate) showed a reduced heart length and mass in comparison to control animals fed with fat-reduced diet. By means of TUNEL-method, apoptosis could be detected in cardiomyocytes of verum animals. Activation of PP2C by unsatturated fatty acids could initiate the endothelial dysfunction and cause lipotoxic cyrdiomyopathy.

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Dates
Created: 2007Issued: 2007-06-08Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2007.0340
Faculty
Fachbereich Pharmazie
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
LipoproteinlipaseApoptosisEndothelzellenApoptosePP2CFettsäurenLipoprotein lipasePP2CPP2CFatty acidsCardiomyocytesHerzmuskelzellenEndothelial cells
DDC-Numbers
610
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2007.0340
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Reinbold, Michael: Induktion von Apoptose durch ungesaettigte Fettsaeuren in Zellen des kardiovaskulaeren Systems. : Philipps-Universität Marburg 2007-06-08. DOI: https://doi.org/10.17192/z2007.0340.

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This item has been published with the following license: In Copyright