Untersuchungen zur generellen Stressantwort von Bacillus subtilis nach Einwirkung von Umweltstress und bei niedriger Temperatur
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Philipps-Universität Marburg
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Abstract
Abstract
The alternative sigma factor SigB
controls the expression of the general stress regulon in
Bacillus subtilis. The synthesis of general stress proteins
supplies non-growing cells of Bacillus subtilis with an
unspecific, multiple and precautionary resistance to future
stress.
To understand the function and interconnection of the
general stress response in the adaptation network of Bacillus
subtilis, DNA-macroarrays were used to monitor the changes in
the transcriptome of cells exposed to heat, salt or ethanol.
This macroarray contains PCR-products of all 4107 annotated
genes of this Gram-positive model organism.
In total 124
SigB-dependent genes were identified containing 62 previously
known SigB-depending genes and 62 genes newly assigned to the
SigB-regulon. As another result the induction of the ECF-sigma
factor SigW after 4% salt stress was shown. In dot blot
experiments the induction of the SigW-regulon after salt stress
was osmotically controlled.
Additionally, the induction of the
general stress response was monitored with lacZ- and
gfp-reporter fusions during the growth at low temperature. In
contrast to the transient induction pattern after environmental
stress the induction during the continuous growth at low
temperature was continuous in exponential growing cells.
The
analysis of different regulator mutants of the SigB-activity
confirmed the temperature sensitive phenotype of the
sigB-mutant and revealed the growth defect of the rsbP- and
rsbUP-mutants due to the weak induction of general stress
proteins in these cells.
A deletion of the rsbV-gene in the
rsbUP-mutant recovered the full induction of the used
gfp-reporter fusion. This rsbUPV-triple mutant also had no
growth sensitive phenotype.
The high and continuous induction
of the general stress response supports the hypothesis of a new
signal transduction pathway to activate SigB in the
cold.Abstract
The alternative sigma factor SigB
controls the expression of the general stress regulon in
Bacillus subtilis. The synthesis of general stress proteins
supplies non-growing cells of Bacillus subtilis with an
unspecific, multiple and precautionary resistance to future
stress.
To understand the function and interconnection of the
general stress response in the adaptation network of Bacillus
subtilis, DNA-macroarrays were used to monitor the changes in
the transcriptome of cells exposed to heat, salt or ethanol.
This macroarray contains PCR-products of all 4107 annotated
genes of this Gram-positive model organism.
In total 124
SigB-dependent genes were identified containing 62 previously
known SigB-depending genes and 62 genes newly assigned to the
SigB-regulon. As another result the induction of the ECF-sigma
factor SigW after 4% salt stress was shown. In dot blot
experiments the induction of the SigW-regulon after salt stress
was osmotically controlled.
Additionally, the induction of the
general stress response was monitored with lacZ- and
gfp-reporter fusions during the growth at low temperature. In
contrast to the transient induction pattern after environmental
stress the induction during the continuous growth at low
temperature was continuous in exponential growing cells.
The
analysis of different regulator mutants of the SigB-activity
confirmed the temperature sensitive phenotype of the
sigB-mutant and revealed the growth defect of the rsbP- and
rsbUP-mutants due to the weak induction of general stress
proteins in these cells.
A deletion of the rsbV-gene in the
rsbUP-mutant recovered the full induction of the used
gfp-reporter fusion. This rsbUPV-triple mutant also had no
growth sensitive phenotype.
The high and continuous induction
of the general stress response supports the hypothesis of a new
signal transduction pathway to activate SigB in the
cold.
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Dates
Created: 2003Issued: 2003-12-23Updated: 2011-08-10
Faculty
Fachbereich Biologie
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
general stress response , Bacillus subtilisSigBGenerelle Stressantwort , Bacillus subtilisSigB
DFG-subjects
Stressproteine , StressorStressreaktion
DDC-Numbers
570
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Brigulla, Matthias (128624132): Untersuchungen zur generellen Stressantwort von Bacillus subtilis nach Einwirkung von Umweltstress und bei niedriger Temperatur. : Philipps-Universität Marburg 2003-12-23. DOI: https://doi.org/10.17192/z2003.0656.
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This item has been published with the following license: In Copyright