Herstellung und Charakterisierung mesenchymaler Stammzellen bei Patienten nach allogener Blutstammzelltransplantation
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Philipps-Universität Marburg
Abstract
Mesenchymal stem cells (MSC) obtained from human bone marrow have been described
as adult stem cells with the ability of extensive self-renewal and clonal
expansion, as well as the capacity to differentiate into various tissue types
and to modulate the immune system. Some data indicate that leukapheresis
products may also contain non-hematopoietic stem cells, as they occur in whole
bone marrow transplantation (BMT). However, there is still controversy whether
MSC expand in the host after transplantation like blood progenitor cells do.
Therefore, we were interested in finding out if graft MSC can be detected in
leukapheresis products and in bone marrow after BMT and peripheral blood stem
cell transplantation (PBSCT). Every sample from total bone marrow transplants
exhibited growth of MSC after in vitro culture, but not one of nine
leukapheresis products did. In addition, bone marrow aspirates of 9 patients
receiving BMT and of 18 patients after PBSCT were examined for origin of MSC.
Almost all MSC samples exhibited a complete host profile, whereas peripheral
blood cells were of donor origin. We conclude that even if trace amounts of MSC
are co-transplanted during PBSCT or BMT, they do not expand significantly in the
host bone marrow.
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This item has been published with the following license: In Copyright