Einfluss einer kontrollierten Stabilisatorzuführung während der Spende auf die Lagerungseigenschaften leukozytendepletierten Vollblutes
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Philipps-Universität Marburg
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Abstract
Background: It is unclear whether maintaining the correct whole blood-to-anticoagulant (WB : AC) ratio during collection can improve the quality of red blood cell (RB.C)-containing blood products to a clinically relevant degree.
Study design and methods: A total of 2 x 20 CPDA-1 leukoreduced whole blood units suspended in CPDA-1 were investigated. In one group, the anticoagulation was continuously added to the donated blood, maintaining the correct WB : AC ratio during collection, using a new drawing device (MacoPharma ABC®). In the other group, WB units were produced conventionally. ATP, 2,3-DPG, free Hb, K+, glucose, lactate, pH and variables of coagulation were determined on Days 1, 7, 21, 35, 42, and 49 of storage. Variables of RBC deformability and aggregability were determined using a laser-assisted optical rotational cell analyzer (LORCA™).
Results: The ABC and the conventional group showed comparable unit volumes of 525 (SD 5,3) ml versus 524 (SD 10,2) ml and Hb content of 65,9 (SD 5,1) g/unit versus 67,5 (SD 7,8) g/unit, but higher variation after conventional blood drawing (p = 0,006 and p = 0,07, respectively) was observed. During storage, none of the measured quality variables were significantly different between the groups, but in the conventional group there was a higher variation for fHb and K+ (p = 0,04), as well a tendency for a higher variation in the glucose-concentration (p = 0.07). Mean (SD) ATP was 2,33 (0,41) µmol/g Hb versus 2,24 (0,39) µmol/g Hb after 42-day storage. Deformability was not different (p = 0,44), whereas the extent of the aggregation was higher in the conventional group.
Conclusion: The ABC device provided a better standardized blood product but did not improve RBC storage variables or plasma quality. Excess anticoagulant CPDA-1 at the beginning of a donation appears not to significantly affect RBC storage in conventional blood drawing. It is unclear whether the lower degree of aggregation in the ABC-Group means to be of any advance for the recipients or may even has adverse effects.
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Dates
Created: 2015Issued: 2015-12-16
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
Blutsammlungsschadenlesion of collectionwhole blooderythrocyte aggregationerythrocyte deformabilityAggregationBlutlagerungsschadenlesion of storageBlutströmungErythrozytenVerformbarkeitGerinnung
DFG-subjects
VollblutBluttransfusionRheologieBlutspende, Koagulation
DDC-Numbers
610
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Lukow, Christian: Einfluss einer kontrollierten Stabilisatorzuführung während der Spende auf die Lagerungseigenschaften leukozytendepletierten Vollblutes. : Philipps-Universität Marburg 2015-12-16. DOI: https://doi.org/10.17192/z2015.0598.