Item type:Thesis, Open Access

CAR-T-Zellen erkennen und eliminieren den Thyreotropin-Rezeptor exprimierende differenzierte Schilddrüsenkarzinomzellen

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2020-03-30

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Philipps-Universität Marburg
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Abstract

Background: Differenciated thyroid cancer presents with a very favourable prognosis. However, there are some cases with differentiated thyroid cancer that are refractory to the established treatment consistant of thyroidectomy, radioactive 131-Iodide therapy and Thyrotropin-suppression. Chimeric antigen receptor T-cells (CART) have already shown impressive results in hematological cancers. Objective: This study aims to evaluate the potential of CART targeting the Thyrotropin-receptor (anti-TSHR CART) against differentiated, TSHR-positive thyroid cancer. Material and methods: Target cells are the papillary thyroid cancer cell line K-1 and the follicular thyroid cancer cell line FTC-133. The killing of target cells by anti-TSHR CART is measured as a reduction of target cells in co-culture un-der the microscope, the fluorescence microscope (after GFP-labelling) and with a caspase-3/7-luminescnece analysis. Results: With K-1 cells we could detect a significant difference between anti-TSHR CART and unspecific CART (T-value = 0.01), while anti-TSHR CART in comparison to unmodified T cells just missed significance (T-value = 0.06). With FTC-133 cells we could detect a significant difference between anti-TSHR CART and unmodified T cells (T-value = 0.02), while anti-TSHR CART in com-parison to unspecific CART just missed significance (T-value = 0.06) (signifi-cance level α = 0.05). Conclusion: Anti-TSHR CART pose a potentially new treatment option to ab-late healthy and malignant transformed thyroid cells expressing the Thyrotropin-receptor.

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Dates
Created: 2020Issued: 2020-03-30Updated: 2020-03-30
DOI
https://doi.org/10.17192/z2020.0170
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
CAR-T-ZellenCAR T cells
DFG-subjects
Immuntherapie
DDC-Numbers
610
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2020.0170
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Spieker, Nicola: CAR-T-Zellen erkennen und eliminieren den Thyreotropin-Rezeptor exprimierende differenzierte Schilddrüsenkarzinomzellen. : Philipps-Universität Marburg 2020-03-30. DOI: https://doi.org/10.17192/z2020.0170.

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This item has been published with the following license: In Copyright