Item type:Thesis, Open Access

Der humane Transporter EAAT 3 ist ein Kandidat für die Vermittlung der L-Glutamat-Aufnahme in Plasmodium falciparum-infizierte Erythrozyten

Thumbnail Image

Files

dmw.pdf (3.02 MB)

Date

2009-06-30

relationships.isAuthorOf

Publisher

Philipps-Universität Marburg
DOI

item.page.supervisor-of-thesis

Abstract

The intracellular parasite Plasmodium falciparum, the causative agent of Malaria tropica, invades human erythrocytes. After invasion the parasite is largely hidden from immune recognition but confronted with a limited nutrition supply. Therefore the parasite induces an increase in solute transport across the host plasma membrane, the so-called novel permeation pathways (NPP). These alterations in the permeability of the erythrocyte plasma membrane assure the supply of nutrients and the disposal of waste products. This study investigates the mechanisms involved in transport of amino acids across the plasma membrane of infected human erythrocytes. In this context it was shown that treatment with sodium arsenite induces NPP-like permeability in non-infected erythrocytes. The induced permeability is limited to negatively charged amino acids and shows characteristics of an EAAT-mediated transport. The influx of glutamate and aspartate into the arsenite-treated erythrocytes is sodium dependent, stereoselective for L-glutamate and sensitive to known EAAT-specific Inhibitors like cis-ACBD and HIP-A. These properties can also be found in Plasmodium-infected cells, suggesting that an EAAT-type transporter is involved in formation of the NPP. Moreover this study provides evidence that the NPP comprise at least three independent pathways for amino acids.

Review

Metadata

Contributors
Supervisor:
Dates
Created: 2009Issued: 2009-06-30Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2009.0132
Faculty
Fachbereich Biologie
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
Novel Permeation PathwaysEAAT3NatriumarsenitNPPL-GlutamatGlutamattransporterSodium arsenite
DFG-subjects
ErythrozytPlasmodium falciparumMalaria tropica
DDC-Numbers
570
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2009.0132
show more
Winterberg, Markus (138390967): Der humane Transporter EAAT 3 ist ein Kandidat für die Vermittlung der L-Glutamat-Aufnahme in Plasmodium falciparum-infizierte Erythrozyten. : Philipps-Universität Marburg 2009-06-30. DOI: https://doi.org/10.17192/z2009.0132.

License

item.page.license.under-copyright
This item has been published with the following license: In Copyright