Effektivität der Keimreduktion durch 445nm Laserbestrahlung parodontaler Taschen in vitro
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Philipps-Universität Marburg
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Abstract
At present, diode lasers are used mainly in periodontitis therapy in the form of antimicrobial photodynamic therapy (aPDT). A photosensitizer is applied to the sulcus and activated by the diode laser. The aim of the present study was to find out to what extent the use of diode lasers without the additional use of a photosensitizer is suitable for disinfecting periodontal pockets and reducing the bacterial germ spectrum within the gingival pocket.
For this purpose, two diode lasers (SiroLaser: Denstply Sirona, Bensheim, Germany; and Aquamarine Laser: FONAdental, Slovakia) in different power levels (0.4W, 0.6W and 0.75W) were subject to a digital power measurement in order to verify the actual laser power. Then titanium rods, previously inoculated with a specific paropathogenic germ spectrum, were placed in a prepared gingival pocket of a pig's mandible and irradiated for 30 seconds with the respective power level of the diode laser. As a reference value, rinsing with 0.2% Chlorhexidine (CHX) rinsing solution for 30 seconds was carried out in a further test group. Another test group represented a sham application of the diode laser (laser power 0W), as well as a control group of untreated titanium rods. The residual germ load on the treated test strips was then determined by an external laboratory (Orodentale Mikrobiologie, Kiel).
The evaluation of the total bacterial load on the treated titanium rods showed, when comparing the five test groups (n = 16), a significantly higher germ reduction in all three test groups in which laser disinfection took place. The lowest residual germ load was achieved by the laser with 0.75W and a wavelength of 970nm (median: 1.1E + 06), closely followed by the 0.6W laser with a wavelength of 445nm (median: 1.4E + 06) and the 0.4W laser with a wavelength of 970nm (median: 2.9E + 06) . The test group with CHX rinsing solution showed a significantly higher bacterial load (median 7.1E + 06). The control group (median 2.7E + 07) and the group in which the laser was used when switched off (median 2.6E + 07) showed no statistically relevant differences.
The results of the present study show that a germ reduction by diode lasers of wavelengths 445nm and 970nm is possible. A direct correlation between the amount of laser power used and the amount of germ reduction could not be conclusively proven and requires further investigations. The study shows that the germ reduction with all three power levels of the diode laser is significantly higher than with conventional rinsing with Chlorhexidin solution.
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Created: 2024Issued: 2025-01-08Updated: 2025-01-08
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
parodontale LaserbestrahlungaPDTadjuvant germ reductionDiode laserperiodontal laser irradiationadjuvante Keimreduktion
DFG-subjects
Halbleiterbakterielle ReduktionDiodenlaserChlorhexidinspülungparodontale TascheaPDTsubgingivale bakterielle Belastung
DDC-Numbers
610
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Lulic, Martin: Effektivität der Keimreduktion durch 445nm Laserbestrahlung parodontaler Taschen in vitro. : Philipps-Universität Marburg 2025-01-08. DOI: https://doi.org/10.17192/z2024.0387.