Item type:Thesis, Open Access

Expression und Regulation von Typ II transmembranären Serin Proteasen (TTSP) auf humanen intestinalen Epithelzellen

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Date

2010-01-26

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Philipps-Universität Marburg
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Abstract

Type II transmembrane serine proteases (TTSP) are a new and expanding family of proteolytic enzymes. Currently little is known about their physiological functions, although it was shown that many TTSP are overexpressed in human tumors. In this study the expression and regulation of four TTSP (namely MT-SP1, Hepsin, TMPRSS-2, TMPRSS-4) on human intestinal epithelial cells using a real-time PCR was rewied. It could be shown that TTSP and PAR-2 are coexpressed in human gastrointestinal tissues and this with highest levels around coecum. In further inflammatory cell model studies using cytokines and different bacterial strains TTSP expression was detected. Interestingly Entercoccus faecium induced a considerable downregulation of expression. In contrast E. coli Nissle as a probiotic strain induced a strong overexpression. Different preparations of E. coli exhibit that the heat-inactivated preparation induced highest overexpression.

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Dates
Created: 2009Issued: 2010-01-26Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2009.0688
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
Bakterieniec cultureIECGene expressionBacteriaZytokineTTSPCytokineTransmembrane protease
DFG-subjects
SerinproteinasenGastrointestinaltraktGenexpressionHT29-Zelle
DDC-Numbers
610
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2009.0688
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Siebert, Marcus Rudi: Expression und Regulation von Typ II transmembranären Serin Proteasen (TTSP) auf humanen intestinalen Epithelzellen. : Philipps-Universität Marburg 2010-01-26. DOI: https://doi.org/10.17192/z2009.0688.

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