Einfluss von verschiedenen Lagerungsmedien auf Biolumineszenzmessungen – eine In-vitro-Studie
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Date
2025-10-21
Authors
Publisher
Philipps-Universität Marburg
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Abstract
Aim: The aim of this study was to investigate the bioluminescence behaviour on
carious lesions after a storage in different storage media over a period of four weeks.
Furthermore, the correlation between the bioluminescence measurements using the
CALCIVIS® Caries Activity Imaging System and the visual findings of the
International Caries Detection and Assessment System was examined.
Material and methods: In total 72 extracted human posterior teeth of the permanent
dentition were carefully cleaned and categorized according to the criteria of the ICDAS
codes. Eight teeth each of the same ICDAS code were stored in one of the three storage
media A = distilled water, B = chloramine-T and C = freezing at -20° C. The teeth were
measured by CALCIVIS (yes/no decision) at baseline, after one week and after four
weeks. The determined data was inserted into MS Excel and analyzed with MedCalc
19.1.7. The correlation between the findings from both systems were listed in cross
tables and evaluated by Spearman's rank correlation coefficient (rs). Pixel values were
calculated in a defined area to quantify the bioluminescence fields over time.
Measurement differences of pixel values between and within the bearing groups were
determined by the Kruskal-Wallis test and the Friedman test. The results were
presented in box plots.
Results: The visual findings of the ICDAS correlated significantly in a high range with
the measured values from CALCIVIS (yes/no decision) for the used 72 teeth (rs =
0.86). Also within the individual storage groups significant correlations could be
validated for A = distilled water (rs = 0.83), B = chloramine-T (rs = 0.77) and C =
freezing at -20° C (rs = 1.0). The evaluation of the Kruskal-Wallis test shows no
significant differences between the groups at the time of baseline (p = 0.27). The
measured values of the cross tables for recording the enamel surface activity according
to ICDAS and CALCIVIS at the time of baseline confirm the results of the Kruskal-
Wallis test once again. Both methods show a high level of correlation for detecting
carious lesions: for all 72 teeth = 90.9 %, group A = 92.9 %, group B = 81.2 % and
group C = 100 %. The measured values for the detection of inactive lesions also
showed a high correlation: for all 72 teeth = 94.6 %, group A = 90 %, group B = 100 %
and group C = 100 %. Significant differences could be measured after one week (p <
0.0001) and four weeks (p < 0.032). After one week all measured values of the three storage groups differed from each other. The measurements after four weeks revealed
differences between group A and C. For the evaluation of the Friedman test there were
no significant differences in the pixel values for storage group A = distilled water over
the time (p = 0.33). For the storage group B = chloramine-T significant differences in
pixel values over time could be validated (p < 0.0001). The pixel values in group B
differed from A and C. Additionally significant differences in the pixel values over
time could be evaluated for the storage group C = freezing at -20° C (p < 0.0001).
Discussion and conclusion: The results of this study between the visual findings
according to the ICDAS and the bioluminescence measurements correlated
significantly and showed a high diagnostic accordance between the two systems. The
bioluminescence method can therefore be used in caries detection as an aid to current
clinical methods such as ICDAS. With regard to the storage of extracted teeth for
examining the bioluminescence behavior distilled water (group A) was best suited in
this study. The storage of extracted teeth in chloramine-T or freezing them at -20° C is
not recommended.
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Supervisor:
Dates
Created: 2024Issued: 2025-10-21Updated: 2025-10-21
Faculty
Medizin
Language
ger
Data types
DoctoralThesis
DFG-subjects
CalcivisLagerungsmediumBiolumineszenz
DDC-Numbers
610
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Davidoff, Daniel: Einfluss von verschiedenen Lagerungsmedien auf Biolumineszenzmessungen – eine In-vitro-Studie. : Philipps-Universität Marburg 2025-10-21. DOI: https://doi.org/10.17192/z2025.0456.
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This item has been published with the following license: In Copyright