Systematische Strukturvariationen oligoaminmodifizierter Peptide, deren Synthese und biologische Anwendung
Loading...
Files
Date
relationships.isAuthorOf
Publisher
Philipps-Universität Marburg
item.page.supervisor-of-thesis
Abstract
Within the scope of this work, various synthetic-strategic approaches for the production of complex oligoamines and modified lysine derivatives have been developed. Irrespective of the synthetic pathway pursued, all of the planned target molecules are cationic compounds, which are among the most important components of numerous proteins and other nitrogen-containing biomolecules. The uniqueness of the amino compounds to be synthesized is certainly due to their structural diversity, which is also based on their biological activity and their astounding presence. The nature-inspired cationic structural motifs allow you to link different independent topics. For example, macromolecules with a defined degree of oligomerization were prepared for investigating the folding behavior of the prion protein. By systematically altering the charge pattern by the incorporation of differently methylated lysine building blocks, important conclusions could be drawn regarding the structure-affinity-activity relationships between the synthetic PrP peptides and naturally occurring PrP autoantibodies. On the basis of the findings on the biochemical processes of biomineralization, this work also elaborated efficient synthetic approaches which allow access to the naturally occurring organic components with silica-precipitating properties. By means of the synthetic polyamines, further details with the regard to their structure-directing role on the precipitation behavior of silicic acid should be obtained. For solid state NMR experiments, specifically labeled oligoamines were prepared. Since most of the in vitro experiments, carried out so far for chemoselective silification, have always been based on the analysis of the precipitated SiO2, efforts should be made within this work to elucidate the dynamics of the silification process by means of NMR spectroscopy.
Therefore, the polyamine-modified prion peptides serve as the basis for the research of protein deficiency diseases, while the synthetic oligoamines or polyamine-based natural products will provide important insights into the biomineralization process.
Review
Metadata
Contributors
Supervisor:
Dates
Created: 2017Issued: 2017-12-14Updated: 2017-12-14
Faculty
Fachbereich Chemie
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
chemoselektive Kieselsäurefällungpost-translational modification of the lysine sidesynthetiSynthese isotopenmarkierter langkettiger PolyamineSynthesis of isotopically labeled long-chain polyamines,c hemoselective silicic acid precipitationPosttranslationale Modifikation der Lysin-Seitenkette
DDC-Numbers
540
show more
Abacilar, Maryna: Systematische Strukturvariationen oligoaminmodifizierter Peptide, deren Synthese und biologische Anwendung. : Philipps-Universität Marburg 2017-12-14. DOI: https://doi.org/10.17192/z2017.0244.
License
This item has been published with the following license: In Copyright