The innate immune system recognizes microbial components (pathogen associated molecular patterns) of Gram-positive and Gram-negative bacteria by means of pattern recognition receptors which lead to the activation of certain defence mechanisms. The family of Toll-like receptors have a crucial role in immune defence and are expressed on different cell types like epithelial cells and fibroblasts. TLR9 seems to play a pivotal role in periodontal diseases by recognizing double-stranded bacterial DNA of periodontopathogenic bacteria, which as PAMP can cause production of proinflammatory cytokines and in case of delayed or missing therapy can lead to chronic inflammation and destruction of the supporting structures of teeth.
In order to examine the immunological effects in the context of periodontitis, this work focused on analyzing in vitro proinflammatory cytokine secretion by human gingival fibroblasts upon stimulation with different TLR9-ligands. HEK 293 cells transfected with TLR9 and human PBMCs were used as reference cell cultures. This work mainly aimed to investigate whether suppressive oligodeoxynucleotides like G-ODN can inhibit TLR9-mediated activation of innate immunity by bDNA.
In summary, the results reveal different stimulatory patterns of the diverse cell systems used, wherein all stimulations could significantly be inhibited by means of suppressive oligodeoxynucleotides.
The findings were put in context with mucosal immunity and the therapeutical application of G-ODN in bDNA-mediated periodontitis was discussed.
Plag, Donatha (1056825723): Zur Pathogenese polymikrobieller Infektionen am Beispiel der Parodontitis. : Philipps-Universität Marburg 2014-09-08. DOI: https://doi.org/10.17192/z2014.0574.
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