Item type:Thesis, Open Access

Ermittlung der m-RNA-Expressionsmuster der c-jun, c-fos und c-myc Proto-Onkogene nach ex vivo Ballondilatation von Schweinekarotiden mittels semiquantitativer RT-PCR.

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Date

2006-08-16

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Philipps-Universität Marburg
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Abstract

The goal of the present research study is to investigate the m-RNA-patterns of the proto oncogenes c-jun, c-myc and c-fos after balloon dilatation by semiquantitative RT-PCR in a porcine model. We generated successfully a simple and applicable method for this study. This method allowed us to determine short sequences of porcine c-myc and c-jun gene. We could not detect any change in m-RNA patterns due to a too small number of vessels used in this study. Nevertheless we saw a reduction in m-RNA expression of c-jun and an increase in m-RNA expression of c-fos after balloon dilatation. We could not find the so far describes increase in c-jun and c-myc gene expression. Additionally we could proof by a histological preparation of the vessels that dilatated and control-vessels are not damaged by dilatation and preparation. Further all vessel walls were found to be intact. There was no proof of denudation.

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Dates
Created: 2005Issued: 2006-08-16Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2006.0563
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
Gene expressionRT-PCRProto-oncogenesProto-OnkogeneRT-PCR
DFG-subjects
Genexpression
DDC-Numbers
610
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2006.0563
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Steinhagen, Sandra: Ermittlung der m-RNA-Expressionsmuster der c-jun, c-fos und c-myc Proto-Onkogene nach ex vivo Ballondilatation von Schweinekarotiden mittels semiquantitativer RT-PCR.. : Philipps-Universität Marburg 2006-08-16. DOI: https://doi.org/10.17192/z2006.0563.

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