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Identifizierung des zentralen Polypeptids des generellen RNA-Polymerase III Transkriptionsfaktors TFIIIC1

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Date

2004-08-12

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Philipps-Universität Marburg
DOI

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Abstract

The human transcription factor TFIIIIC1 is a general factor for transcription of all RNA pol III dependent genes. There are no information about the molecular entity of TFIIIC1. The aim of this work was the purification of TFIIIC1 by chromatography in order to analyse the molecular entity of TFIIIC1 by MALDI TOF. The use of anionic exchanger MiniQ resulted in purification and conzentration of TFIIIC1 fractions which was nesessary for further purification of TFIIIC1 by size exclusion chromatography (SEC). SEC resulted in MALDI TOF analysis of seven polypeptides of the most purified TFIIIC1 fraction. Western blot analyses and immundepletion identified the "Transcription Factor-like Nuclear Regulator" (TFNR) as the central polypeptid of TFIIIC1. The 250 kDa protein TFNR shows N-terminal homology to the 150-160 kDa protein hBdp1 (hB'', TFIIIB150). Bdp1 is discribed as an essential pol III transcription factor and the third component of TFIIIB. The identification of the central polypeptid of TFIIIC1 activity proof the function of the 250 kDa form of hBdp1 (TFNR) as essential pol III transcription factor. The N-terminus of hBdp1 is the essential part for transcription activity in vitro.

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Supervisor:
Dates
Created: 2004Issued: 2004-08-12Updated: 2011-08-10
Faculty
Medizin
Language
ger
Data types
DoctoralThesis
Keywords
TFNRBdp1Bdp1TFIIIBTFNRTFIIIC1TFIIIC1TFIIIB
DFG-subjects
RNS-Polymerase IIITranskriptionsfaktorTranskription Säulenchromatographie
DDC-Numbers
610
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2004.0420
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Gruber, Christin: Identifizierung des zentralen Polypeptids des generellen RNA-Polymerase III Transkriptionsfaktors TFIIIC1. : Philipps-Universität Marburg 2004-08-12. DOI: https://doi.org/10.17192/z2004.0420.

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