Stellenwert der Mismatch-Repair Gene in der Ausbildung der Chemorefraktarität bei testikulären Keimzelltumoren
Loading...
Files
Date
relationships.isAuthorOf
Publisher
Philipps-Universität Marburg
item.page.supervisor-of-thesis
Abstract
The above thesis is of experimental character. This thesis combines elements of urology, oncology, pathology and immunohistochemistry. It was done under supervision of Professor Dr. med. Axel Heidenreich formerly assistant medical director of the urological department at the University Hospital at Philipps University Marburg, now head of the uro-oncological department at the University Hospital at the University of Cologne
Testicular cancer is the leading solid malignancy in young men between the age of 20 to 40 years. Because of the great advances in chemotherapy the mean overall survival rate is up to 90%, there is still a small percentage of about 6% of patients who will die because of chemoresistancy to the two most common agents, cisplatin and etoposid. Another 14% of patients show active tumorcells in removed lymph nodes by RPLA, 30-40% show chemoresistant teratoma.
A study of literature indicates that mismatch repair genes such as hMSH2 as well as hMLH1 (MMR) play a key role in the development of cisplatin and etoposid associated chemoresistancy in tumors such as ovarian cancer.
MMR induce apoptosis through the c-Jun and c-Abl pathway.
The goal of my thesis was to investigate the status of MMR through immunohistochemistry in patients with refractory testicular germ-cell cancer.
I tested the primary testicular tumor specimens as well lymphnodes from RPLA after inductive chemotherapy.
The specimens were tested for hMLH1 and hMSH2 expression.
Results:
In my thesis I was able to demonstrate that about 48% of the tested chemoresistant tumors had a loss of either hMLH1 or hMSH2. 37% just had a loss of hMLH1 and 11% only had a loss of hMSH2. These results correlate with the results of another study group, Mayer et al. Mayer et al. were able to demonstrate a connection between microsatellite instability (MSI) and chemoresistancy in a smaller number of samples. A loss of MMR is characterized my Microsatellite Instability.
Because of the greater number of samples in this thesis it is possible to connect the loss of MMR with the development of chemoresistancy in testicular germ-cell tumors.
Review
Metadata
Contributors
Supervisor:
Dates
Created: 2007Issued: 2007-10-29Updated: 2011-08-10
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
Chemotherapyresistence
DFG-subjects
Therapieresistenz
DDC-Numbers
610
show more
Peitgen, Elena: Stellenwert der Mismatch-Repair Gene in der Ausbildung der Chemorefraktarität bei testikulären Keimzelltumoren. : Philipps-Universität Marburg 2007-10-29. DOI: https://doi.org/10.17192/z2007.0715.
License
This item has been published with the following license: In Copyright