Vergleich von zwei Geräten und zwei Atemmustern bei der Gewinnung von exhaliertem Atemwegskondensat
Loading...
Files
Date
Authors
Publisher
Philipps-Universität Marburg
Supervisors
Abstract
EBC collection (Exhaled breath condensate) is a promising non-invasive methodto obtain samples from the epithelial lining fluid of the lung. Due to
problems of standardization concerning methodical aspects EBC analysis is
presently not used in clinical practice. For the first time the comparability for the two commercially available devices (RTube™ and ECoScreen Turbo) was assessed in healthy controls by measuring pH, conductivity and total protein.
A further aim of the study was to assess the influence of different
breathing patterns (tidal breathing and hyperventilation) on total protein
amount and specific proteins (Surfactant Protein-A, das Club Cell Protein,
Alpha-1-Antitrypsin) in EBC. EBC was collected in different experimental
setup (≥ 10 individuals each) using the RTube™ and ECoScreen Turbo in
a randomized crossover design, twice with every device - once in tidal breathing and once in hyperventilation. It could be shown that neither the device nor the breathing pattern significantly altered EBC pH or conductivity.
Total protein measurements however were altered by breathing patterns. It
could be shown that hyperventilation increased total protein concentrations
in EBC. To analyse the source of EBC the bronchial Club Cell Protein (CCP)
and Surfactant protein A (SP-A) as a marker of the alveolar fraction were
measured after tidal and breathing and hyperventilation. However SP-A and
CCP were not consistently detectable in EBC. Thus the question concerning
the origin of EBC cannot be answered satisfying at the moment. Alpha-1
Antitrypsin (AAT) was consistently detectable in EBC using both the RTube
™ and the ECoScreen Turbo. It was the first time that AAT was measured
in EBC collected with den ECoScreen Turbo. It could be shown that AAT
concentration was not statistic significantly altered by the device. Instead
hyperventilation increased –similar to the measurements of total protein-
AAT concentration in EBC. Up to now there is no research work that would
have investigated AAT concentration in EBC after hyperventilation. Finally
it could be shown that results of protein measurements changed significantly
after normalizing the absolute amount or concentrations of total protein
and AAT to ventilated air. Therefore standardization to the ventilated air is recommended for future protein analysis in EBC.
Review
Metadata
Contributors
Supervisor:
Dates
Created: 2017Issued: 2017-11-17Updated: 2017-11-27
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
Exhaled breath condensatedevicephAATbreathing patternstotal protein
DFG-subjects
AtemwegskondensatGerätevergleichAATpHAtemmuster Gesamtproteinkonzentration
DDC-Numbers
610
show more
Schober, Eva-Maria: Vergleich von zwei Geräten und zwei Atemmustern bei der Gewinnung von exhaliertem Atemwegskondensat. : Philipps-Universität Marburg 2017-11-17. DOI: https://doi.org/10.17192/z2017.0727.
License
This item has been published with the following license: In Copyright