Item type:Thesis, Open Access

Der verstärkende Effekt einer Laserphotochemotherapie mit Cisplatin oder Zolendronsäure bei Knochen- und Knochentumorzellen in-vitro.

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Date

2017-12-20

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Philipps-Universität Marburg
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Abstract

Nowadays, the low level laser therapy ist used as locally conservative therapy in medical applications, by leading to promote wound healing and reduce inflammation. This is done by a direct effect of the biostimulative laser irradiation on the mitochondria. This process is referred to as Photobiomodulation. Photodynamic therapy (PDT) is based on a combined using of a photosensitizer and the laser irradiation. In dermatology it is used for the treatment of skin lesions. In addition, it is used in dentistry in the treatment of root canals, periodontitis and periimplantitis as antimicrobial photodynamic therapy. This study aims to study the effect of a combination of LLLT with chemotherapy (cisplatin or zoledronic acid) in vitro on cellular metabolism. For this purpose, primary human osteoblasts and bone tumor cells (Saos-2 cell line) were incubated with different concentrations of cisplatin or zolendronic acid and then were irradiated twice with a diode laser (670 nm, 2 minutes, 100mW/cm^2). This was followed by either XTT assay (cell viability test) or histological examination by HE staining. Compared to the non-irradiated control group, a single laser irradiation increased metabolism of both cell lines. After pretreatment with cisplatin or zoledronic acid the radiation-induced cytotoxicity was clearly recognizable compared to the non-irradiated control. The osteoblasts showed lower photosensitivity for cisplatin compared to Saos-2 tumor cell line. In cases of pretreatment with cisplatin the viability of the two cell lines has been reduced by their simultaneous laser irradiation. The increased radiation-induced toxicity occurred only by the highest concentrations of zoledronic acid. These results were reflected in the histological examination as well. LLLT can lead to an increase of toxicity of local applied chemotherapeutic agent. This can be of therapeutic interest in the deveolopment of new therapeutic approaches for bone tumors and cysts in the oral and maxillofacial region.

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Dates
Created: 2017Issued: 2017-12-20Updated: 2018-01-17
DOI
https://doi.org/10.17192/z2017.0792
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
DFG-subjects
CisplatinZolendronsäureLaserphotochemotherapieKnochenkrankheit
DDC-Numbers
610
License
https://creativecommons.org/licenses/by-nc-nd/4.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2017.0792
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Saydali, Akram: Der verstärkende Effekt einer Laserphotochemotherapie mit Cisplatin oder Zolendronsäure bei Knochen- und Knochentumorzellen in-vitro.. : Philipps-Universität Marburg 2017-12-20. DOI: https://doi.org/10.17192/z2017.0792.

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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 - CC BY NC ND
Except where otherwised noted, this item's license is described as Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 - CC BY NC ND