Item type:Thesis, Open Access

Die invasive Front von Plattenepithelkarzinomen des oberen Aerodigestivtraktes exprimiert MMP-9 und ist eine bevorzugte Lokalisation von Tumorstammzellkandidaten

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Date

2011-02-18

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Philipps-Universität Marburg
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Abstract

Head and neck squamous cell carcinomas (HNSCCs) are the most frequent malignancies of the upper aerodigestive tract. The cancer stem cell (CSC) hypothesis concludes that CSCs constitute the dangerous tumor cell population due to their ability of self-renewal and being associated with relapse of tumor disease, invasiveness and resistance to chemo(radio)therapy. The aim of this study was to look for CSC candidates and expression of MMP-9 that previously was implicated in HNSCC invasiveness. Immunohistochemical, immunofluorescence and Western blot analysis were performed on HNSCC tumor specimens using antibodies specific for MMP-9, CD44, ALDH1 and CK14. Gelatinolytic activity was assessed by zymography. Pearson correlation analysis was used for statistical comparison. Immunohistochemical analysis found CD44 and MMP-9 to co-localize in tumor cells at the invasive front. Western blot analysis demonstrated a significant correlation (p = 0.0047) between CD44 and MMP-9 in the tested tissues. In addition gelatinolytic activity of HNSCC tissues was found to significantly correlate (p = 0.0010) with MMP-9 expression. The CD44+ invasive front of the tumor was also positive for ALDH1 and CK14, all of them being typically expressed by cells in the basal cell layer of normal stratified squamous epithelia that also harbors the epithelial stem cells. The observations point to a role of a MMP-9 positive basal-cell-like cell layer in the process of HNSCC invasiveness. This compartment likely contains CSCs since it is expressing the putative CSC markers CD44, ALDH1 and CK14. This cell layer therefore should be considered a major therapeutic target in the treatment of head and neck cancer.

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Dates
Created: 2011Issued: 2011-02-18Updated: 2011-08-08
DOI
https://doi.org/10.17192/z2011.0028
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
CSCMMPCD44InvasivenessHNSCCALDH1
DFG-subjects
MMP-9InvasivitätStammzelleHals-Nasen-Ohren-Tumor
DDC-Numbers
610
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2011.0028
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Sterz, Carolina: Die invasive Front von Plattenepithelkarzinomen des oberen Aerodigestivtraktes exprimiert MMP-9 und ist eine bevorzugte Lokalisation von Tumorstammzellkandidaten. : Philipps-Universität Marburg 2011-02-18. DOI: https://doi.org/10.17192/z2011.0028.

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This item has been published with the following license: In Copyright