Kompartimente der Weißen Milzpulpa bei Ratten - Immunhistologische Darstellung von Stromazellen, Lymphozyten und Makrophagen
Loading...
Files
Date
Authors
Publisher
Philipps-Universität Marburg
Supervisors
Abstract
The microscopic anatomy of rat spleens has been described by
immunohistology 30 years ago shortly after monoclonal antibody (mAb)
technology came up. Up-to-date surveys of rat splenic cell composition are
lacking. In the present investigation a highly amplifying immunohistological
technique, the ABC method, is applied to visualise stromal cells, B- and T-lymphocytes,
macrophages and plasma cells/plasmablasts in spleens of the
inbred LEW rat strain. Special emphasis is put on analysing cells in the splenic
marginal zone (MZ), a border region between white and red pulp, which has
originally been defined in rats.
The antibodies used confirmed the well-known distribution of B- and T-lymphocytes
in the rat spleen. In addition, two mAbs, His57 and His52, stained
the majority of B-lymphocytes in the MZ and B-cells in follicles. Both mAbs have
been described not to react with small recirculating B-lymphocytes in the
original publication. The staining pattern observed may be due to MZ B-lymphocytes
migrating between MZ and follicles. The present investigation
defines the phenotype of MZ B-lymphocytes in LEW rats as
His57++His52+IgM+IgD+/-.
Stromal cells of the MZ could not be immunohistologically defined in rats. The
phenotype of these cells obviously differs decisively from that of fibroblasts at
the surface of human splenic follicles. Detection of ICAM-1 (CD54) was not
informative, because all MZ B-lymphocytes expressed this adhesion molecule.
In contrast, VCAM-1 (CD106) was almost absent in the MZ. Anti-pan-laminin
showed an extracellular network in the MZ, which appeared to be produced by
stromal cells. This network was also visualised by mAb Ox43. In addition, Ox43
targeted all parts of the open splenic circulation system. However, in other
organs Ox43 produced typical membrane staining of different cell types. The so
far uncharacterised Ox43 antigen could thus be a membrane molecule, which is
also cleaved and circulates in the blood. It may have a coagulation-inhibiting
function.
Plasmablasts and plasma cells with high amounts of intracellular IgM formed
clusters in the outer T-cell zone and near side branches of the central arteries
termed "marginal zone bridging channels". About half of these cells expressed
Ki-67. This suggests that not only proliferating plasmablasts, but also newly
formed non-proliferating plasma cells may migrate in the spleen for longer
distances. It cannot be excluded that IgM+ plasma cells are formed in the outer
T-cell zone.
The MZ clearly belongs to the open splenic circulation, because it harbours
scattered erythrocytes and granulocytes. The presence of MZ B-cells, VCAM-1-
stromal cells and CD169++ macrophages characterises the MZ as a unique
species-specific splenic compartment. Rat MZs contain small recirculating B-lymphocytes
and a predominant population of large pre-activated B-
lymphocytes which most likely correspond to memory B cells. Whether these
cells exhibit species-specific traits similar to the stromal cells is not clear. The
phenotype of MZ stromal cells remains to be established in rats and mice.
Review
Metadata
Contributors
Supervisor:
Dates
Created: 2021Issued: 2023-03-09Updated: 2023-03-09
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
ratimmunologyspleen
DFG-subjects
ImmunologieRatteMilzImmunohistologieMakrophagenStromazellenLymphozyten
DDC-Numbers
610
show more
Mecha, Alissa: Kompartimente der Weißen Milzpulpa bei Ratten - Immunhistologische Darstellung von Stromazellen, Lymphozyten und Makrophagen. : Philipps-Universität Marburg 2023-03-09. DOI: https://doi.org/10.17192/z2023.0134.
License
This item has been published with the following license: In Copyright