Extrazelluläre Vesikel als neue Biomarker bei der Diagnostik entzündlicher Lungenerkrankungen
Loading...
Files
Date
Authors
Publisher
Philipps-Universität Marburg
Supervisors
Abstract
Community-acquired pneumonia (CAP) and acute exacerbated COPD (AECOPD) are
two common diseases associated with high morbidity and mortality worldwide. Due to
similarities in their clinical presentation, differential diagnosis is difficult. Small
extracellular vesicles (sEVs) are considered as potential as new biomarkers. Their
surface proteins and other constituents are depending on their cell of origin and could
be a step toward the anticipated "liquid biopsies".
In this pilot study, we examined the surface proteins of plasma exosomes that possibly
allow the differential diagnosis of CAP and AECOPD. For this purpose, 40 surface
proteins were analysed by EV-array. The study was focused on the potential of the
biomarkers to differentiate between the different study groups. Also, the ability of the
parameters to estimate the severity of pneumonia was studied. Other parameters
collected were blood count and CRP, clinical parameters and clinical scores (CRB-56,
CURB, PSI, GOLD 1-4, GOLD A, B, C, D, and mMRC). In total, plasma samples from
55 human subjects were examined, including 24 CAP patients, 10 AECOPD patients
and 21 healthy subjects.
The EV array revealed significant differences between healthy subjects, CAP patients,
and AECOPD patients. We found CD45 and CD28 to be the best discrimination
markers on plasma exosomes between CAP and AECOPD with an AUC > 0.92 in a
ROC analysis. To distinguish between CAP patients and healthy subjects, CD45 and
CD16 showed the best results. A classification into uncomplicated and severe CAP
was possible with ICAM-1.
Since the diagnosis of CAP in COPD patients is particularly difficult, it was investigated
whether a distinction between CAP and AECOPD is possible even if the CAP patient
had COPD. Here, the ensemble feature selection resulted in a panel consisting of
CD45, CD28, CTLA4, TNF-R-II and CD16.
All in all, the EV array is a simple and minimal-invasive diagnostic tool with potential to
distinguish between healthy subjects, CAP patients, and AECOPD patients. Further
studies with bigger cohorts are needed to explore the potential of sEV proteins as new
biomarkers in the diagnosis of infectious lung diseases.
Review
Metadata
Contributors
Supervisor:
Dates
Created: 2021Issued: 2021-04-21Updated: 2021-04-21
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
extracellular vesiclesexosomesbiomarkerpneumoniaCOPDexosome arrayplasmaacute exacerbation
DFG-subjects
PneumonieExosomenarrayextrazelluläre Vesikelakute ExazerbationPlasmaCOPDBiomarkerExosomen
DDC-Numbers
610
show more
Brinke, Kristina auf dem: Extrazelluläre Vesikel als neue Biomarker bei der Diagnostik entzündlicher Lungenerkrankungen. : Philipps-Universität Marburg 2021-04-21. DOI: https://doi.org/10.17192/z2021.0166.
License
This item has been published with the following license: In Copyright