Item type:Thesis, Open Access

Hypertensive Ratten mit Antibiotika- und G-CSF Prophylaxe überleben eine abdominelle Sepsis besser als Dahl-Salz-sensitive und normotensive Wistar Ratten in klinik-modellierenden randomisierten Studien (CMRTs)

Thumbnail Image

Files

dbt.pdf (906.83 KB)

Date

2006-03-13

Authors

Publisher

Philipps-Universität Marburg
DOI

Supervisors

Abstract

Diabetes mellitus, malnutrition or bloodloss are potential riskfactors which lead to an disturbed immunological reaction against mikroorganismen and to an increased mortality in abdominal sepsis not only in trials with rodens but also in clinical reality. More than this, hypertension is proposed as a risk faktor in the pathogenesis of stroke and heart attack and also infections. In this trial a model of primary hypertension with spontaneous hypertensive rats (SHR) and an intermediate model induced by genetic and enviromental factors with Dahl salt sensitive (DS) rats where chosen for analysis of a G-CSF prophylaxis in rats with polymicrobial abdominal sepsis induced by peritoneal contamination an infection with standarized stool inoculum. Clinical complexity and trial conditions were simulated in clinic modelling ranomized trials (CMRTs). Different mortality rates among the groups (SHR, DS, Wistar) were observed. Hypertensive SHR rats showed a significantly lower mortality rate than normotensive Wistar rats, hypertensive DS rats showed a slight tendency of lower mortality rate than Wistar rats. The proinflammatory cytokines IL 1, IL 6 and MIP 2 in organes of lung, liver and spleen were lower in the SHR group compared to wistar rats. The anti-inflammatory cytokine IL-10 was expressed on a higher level in liver in the strain of SHR with infection compared with Wistar rats. This could be a positive influence for the SHR group whereas the lower expression of IL-10 in DS rats a more neative outcome promotes. Studying the protein levels of circulating cytokines no difference has been found between the strains of SHR and Wistar rats post infection. The local expression of cytokine seem to play a more important role than systemic ones. Hypertension itself is not the decisive factor for cytokin expression and surviving of rats with abdominal infections but rather their genetic bakground. An explanation for the higher resistance and better mortality rate of SHR rats against microbial infection and their produced toxins can be found in a distinct reduced local expression of pro-inflammatory cytokines.

Review

Metadata

Contributors
Supervisor:
Dates
Created: 2005Issued: 2006-03-13Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2006.0201
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
SepsisG-CSFHypertensionDahl-RattenHypertensive RattenDahl-ratsCMRTTierstudienSHR-ratsSHR-Ratten
DFG-subjects
SepsisG-CSFCytokine
DDC-Numbers
610
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2006.0201
show more
Tischer, Björn: Hypertensive Ratten mit Antibiotika- und G-CSF Prophylaxe überleben eine abdominelle Sepsis besser als Dahl-Salz-sensitive und normotensive Wistar Ratten in klinik-modellierenden randomisierten Studien (CMRTs). : Philipps-Universität Marburg 2006-03-13. DOI: https://doi.org/10.17192/z2006.0201.

License

item.page.license.under-copyright
This item has been published with the following license: In Copyright