Item type:Thesis, Open Access

Polyethylenimine and its derivates: investigation of in vivo fate, subcellular trafficking and development of novel vector systems

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Date

2003-12-22

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Philipps-Universität Marburg
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Abstract

In this dissertation several aspects of polymer based gene delivery were investigated. First, key issues in subcellular processing of electrostatic polymer/nucleic acid complexes were investigated and new insights into mechanisms involved in these processes were gained. Secondly, a targeted gene delivery system was developed for the specific transfection of ovarian carcinoma cells. The resulting vector exhibited a high specificity for target cells combined with low unspecific transfection and toxicity. Furthermore, a novel type of gene delivery system was synthesized. This vector exhibited a high in vitro transfection efficiency and a very low in vitro toxicity as well as favourable in vivo properties, such as reduced toxicities. Another aspect that was studied in depth was the investigation of the stability of several electrostatic vectors in vitro and when applied intravenously.

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Dates
Created: 2003Issued: 2003-12-22Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2003.0651
Faculty
Fachbereich Pharmazie
Publisher
Philipps-Universität Marburg
Language
eng
Data types
DoctoralThesis
Keywords
gene therapy , targetingtransfectionsubcellular trafficking , polymer
DFG-subjects
AntikörperGentherapieTransfektionKonjugierte PolymereProzessierung
DDC-Numbers
610
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2003.0651
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Merdan, Thomas (128590513): Polyethylenimine and its derivates: investigation of in vivo fate, subcellular trafficking and development of novel vector systems. : Philipps-Universität Marburg 2003-12-22. DOI: https://doi.org/10.17192/z2003.0651.

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This item has been published with the following license: In Copyright