Kinetik der minimalen Hemmkonzentration verschiedener Antimykotika gegenüber den klinisch isolierten Erregern der Onychomykosen während einer Beobachtungszeit von 20 Wochen
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Authors
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Philipps-Universität Marburg
Supervisors
Abstract
The aim of the investigations was to determine
how long dermatophytes (Trichophyton rubrum and Trichophyton
mentagrophytes) and molds (scopulariopsis brevicaulis) isolated
from patients with a toenail mycosis can survive in sterile
petry dishes, and whether such ex-vivo conditions may change
the susceptibility of the fungi to antimycotics.
Toe nails of
47 patients with onychomycosis were abraised with an electrical
drill. The nail specimens were then stored in sterile petry
dishes for 20 weeks at room temperature. Subsequently after
nail abrasion, and 4, 8, 12, 16 and 20 weeks after abrasion, a
part of the nail specimens were each cultured on 2 different
mediums (Selective- and Standard agar). At week 20 all remained
nail specimens were then cultured. Anytime a growth of fungi
was observed the minimal inhibitory concentrations (MIC) of
amorolfine, ciclopirox, fluconazole, itraconazole and
terbinafine for isolated dermatophytes and molds were assessed
by a microdilution test as described elsewhere
12 strains of
T. rubrum , 5 strains of T. mentagrophytes and 9 strains of
scopulariopsis brevicaulis were isolated from the toe nail of
47 patients with onychomycosis during 20 weeks. At week 20 a
growth of T. rubrum (50%), T. mentagrophytes (40%) and
scopulariopsis brevicaulis (77,8%) could still be proved .
The
MICs of ciclopirox (2-8 µg/ml), amorolfine (0,01-0,5 µg/ml) and
terbinafine (0,005-5 µg/ml) seemed to be stable during the test
period. By contrast, fluconazole (64-2048 µg/ml) and
itraconazole (0,5-40 µg/ml) showed a broad range of the MICs.
This could be explained by a limited reproducibility of the
microdilution test for azole antimycotics. Against
scopulariopsis brevicaulis fluconazole and itraconazole shows
no activity.
Some of dermatophytes (40-50%) and the most
strains of scopulariopsis brevicaulis (77,8%) isolated from
patients with toenail mycosis could survive in sterile petry
dishes at least for a period of 20 weeks. These ex-vivo
conditions do not seem to affect the susceptibility of the
isolated dermatophytes to oral and topical antifungal agents.
Each strain of T. rubrum, T. mentagrophytes and scopulariopsis
brevicaulis showed during the test period an unchanged
susceptibility to the antimycotics. The MICs observed agreed
with previous findings of other groups.The aim of the investigations was to determine
how long dermatophytes (Trichophyton rubrum and Trichophyton
mentagrophytes) and molds (scopulariopsis brevicaulis) isolated
from patients with a toenail mycosis can survive in sterile
petry dishes, and whether such ex-vivo conditions may change
the susceptibility of the fungi to antimycotics.
Toe nails of
47 patients with onychomycosis were abraised with an electrical
drill. The nail specimens were then stored in sterile petry
dishes for 20 weeks at room temperature. Subsequently after
nail abrasion, and 4, 8, 12, 16 and 20 weeks after abrasion, a
part of the nail specimens were each cultured on 2 different
mediums (Selective- and Standard agar). At week 20 all remained
nail specimens were then cultured. Anytime a growth of fungi
was observed the minimal inhibitory concentrations (MIC) of
amorolfine, ciclopirox, fluconazole, itraconazole and
terbinafine for isolated dermatophytes and molds were assessed
by a microdilution test as described elsewhere
12 strains of
T. rubrum , 5 strains of T. mentagrophytes and 9 strains of
scopulariopsis brevicaulis were isolated from the toe nail of
47 patients with onychomycosis during 20 weeks. At week 20 a
growth of T. rubrum (50%), T. mentagrophytes (40%) and
scopulariopsis brevicaulis (77,8%) could still be proved .
The
MICs of ciclopirox (2-8 µg/ml), amorolfine (0,01-0,5 µg/ml) and
terbinafine (0,005-5 µg/ml) seemed to be stable during the test
period. By contrast, fluconazole (64-2048 µg/ml) and
itraconazole (0,5-40 µg/ml) showed a broad range of the MICs.
This could be explained by a limited reproducibility of the
microdilution test for azole antimycotics. Against
scopulariopsis brevicaulis fluconazole and itraconazole shows
no activity.
Some of dermatophytes (40-50%) and the most
strains of scopulariopsis brevicaulis (77,8%) isolated from
patients with toenail mycosis could survive in sterile petry
dishes at least for a period of 20 weeks. These ex-vivo
conditions do not seem to affect the susceptibility of the
isolated dermatophytes to oral and topical antifungal agents.
Each strain of T. rubrum, T. mentagrophytes and scopulariopsis
brevicaulis showed during the test period an unchanged
susceptibility to the antimycotics. The MICs observed agreed
with previous findings of other groups.
Review
Metadata
Contributors
Supervisor:
Dates
Created: 2003Issued: 2003-12-22Updated: 2011-08-10
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
itraconazoleminimal inhibitory concentrationfluconazoleonychomycosisterbinafine, amorolfineciclopirox
DFG-subjects
CiclopiroxolaminOnychomykosisItraconazol , AmorolfinMinimale Hemmkonzentration , TerbinafinFluconazol
DDC-Numbers
610
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Straßmann, Kai (128597003): Kinetik der minimalen Hemmkonzentration verschiedener Antimykotika gegenüber den klinisch isolierten Erregern der Onychomykosen während einer Beobachtungszeit von 20 Wochen. : Philipps-Universität Marburg 2003-12-22. DOI: https://doi.org/10.17192/z2003.0719.
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This item has been published with the following license: In Copyright