The presented work describes the development of new ligand-binding assay platforms for rapid serological diagnosis. 3-D polyethylene sinter bodies and monodisperse gold nanoparticles have been successfully employed as protein carriers, providing sensitive, selective and rapid serological diagnosis. Detection of anti-Borrelia antibodies in human sera samples was taken as a model in our work. Antibodies recognition either by visible observation or by spectroscopic measurements was easily practicable. All developed assays could be performed in a short time, 5-30 min, providing a suitable colorimetric point of care test. The established assays offer simple, rapid and reliable tools of analysis with minimal cost, which can be easily transferred to other infectious diseases.
Alasel, Mohammed: Development of new immunoassay platforms for rapid serological diagnosis of Lyme borreliosis. : Philipps-Universität Marburg 2016-09-07. DOI: https://doi.org/10.17192/z2016.0497.