Der verstärkende Effekt einer Low-Level-Laserbestrahlung auf die Wirkung von Cisplatin und Zolendronsäure in-vitro.
Loading...
Files
Date
relationships.isAuthorOf
Publisher
Philipps-Universität Marburg
item.page.supervisor-of-thesis
Abstract
Today, the biostimulatory effect of low-level laser therapy (LLLT) is mainly used in supportive care of chronic diseases. Beneficial effects in terms of wound healing, tissue reparation, relieving inflammation and as analgesic for neurological disorders have been reported. Furthermore, it is postulated that this kind of radiation as a direct bio-stimulatory effect on mitochondria. This is also named as photobiomodulation. The advancement of this technology is the photodynamic therapy (PDT), where a photosensitizer is activated by light of a specific wavelength. In this described combination, it is used in dental medicine as an antimicrobial photodynamic therapy (aPDT) in the treatment of periodontitis, peri-implantitis or endodontic therapy. In modern dermatology, PDT is widely used against different malignant or non-malignant cutaneous diseases.
The purpose of this in-vitro study was to investigate the effect of LLLT with cisplatin and zolendronic acid as potential photosensitizer on the bioviability of human benign and malignant cells. This could be interesting in the development of new therapeutic options for cancer in the craniomaxillofacial region.
Primary human fibroblasts (PHF) and head and neck squamous cell carcinoma cells (HNSCC-UM-3) were treated with different concentrations of zolendronic acid or cisplatin and were irradiated twice with a non-thermal diode laser (wavelength 670nm, 2 minutes, 100mW/ cm'2). Non-irradiated cells served as control groups. Cell viability was tested by XTT-assay. For histomorphological analysis a H.E. staining was performed.
Laser therapy significantly increased bioviability for both cell lines. For human primary fibroblasts, LLLT increased the cytotoxicity of applied chemotherapeutic substances. When HNSCC-UM-3 cells were incubated with cisplatin, an high photosensibilization was observed in comparison to primary human fibroblasts. In cases of incubation with zolendronic acid, similar to fibroblasts, laser therapy lowered cell viability at the highest concentration only, eventhough zolendronic acid alone was not seen as cytotoxic.
Within the limits of this study, it can be concluded that LLLT is able to enhance the cytotoxicity of local applied chemotherapeutic agents like cisplatin and zolendronic acid. This could be interesting in the development of new therapeutic options using chemotherapeutic drugs as a possible photosensitizer. Possible clinical applications could be the keratocystic odontogenic tumor (KCOT) as well as a supportive tool in terms of metastatic skin cancer, like basal-cell carcinoma, in combination with a systemic chemotherapy.
Review
Metadata
Contributors
Supervisor:
Dates
Created: 2015Issued: 2015-05-28
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
zolendronic acidLLLTciplatin
DFG-subjects
LLLTZolelndronsäureCisplatin
DDC-Numbers
610
show more
Heymann, Paul Günther Baptist (1016277059): Der verstärkende Effekt einer Low-Level-Laserbestrahlung auf die Wirkung von Cisplatin und Zolendronsäure in-vitro.. : Philipps-Universität Marburg 2015-05-28. DOI: https://doi.org/10.17192/z2015.0281.
License
This item has been published with the following license: In Copyright