Auswirkung von O3/O2-Gasgemisch im Peritoneum auf Zytokinproduktion und Schmerzinduktion
Loading...
Files
Date
Publisher
Philipps-Universität Marburg
Supervisors
Abstract
Ozone (O3) is a metastable gas, which disaggregates with the formation of
reactive oxygen combinations. Due to its powerful oxidation ability and
antiseptic qualities, it is often used in human medicine as an additional
therapeutic agent externally. Little is currently known about the pain and
immunomodulational effects of intraperitoneally applied O3.
One aim of this study was the investigation of the pain aspect of
intraperitoneal insufflation of a O3/O2-gas mixture. A further aim was the
collection of evidence of ozone-effects on the immune system.
For the detection and characterization of a possible burden of pain among
intraperitoneal O3/O2-insufflation-therapy we initially performed a writhing
response test. The behavior of C-57BL/6-mice, following intraperitoneal
insufflation of a O3/O2-gas mixture, was compared with that of mice
following intraperitoneal administration of pure oxygen (100% pure O2),
acetic acid (7% H3C-COOH, positive control) or 0.9% NaCl solution
(Sham).
After the writhing test, the mice were decapitated and tissue samples were
taken (including spinal cord and spleen).
As a second step, an analysis was made of a possible pain component of
O3/O2-pneumoperitoneum whereby the c-fos-gene-expression in the
thoracolumbar spinal cord was mesured, using real-time PCR (qPCR).
Furthermore, the effects of ozone on the immune system were
investigated. For this purpose, the change of gene expression of the
proinflammatory cytokines IL-6 and IL-1ß in the secondary lymphatic
mouse spleen tissue was determined by performing another qPCR.
The result of the writhing behavior experiment precludes the occurrence of
visceral pain during intraperitoneal O3/O2-insufflation. By insufflation of
inert O2-gas there was no sign of writhing responses, therefore stretching
artifacts could be excluded. Measurement of significantly increased c-fos-gene-expression in the spinal cord suggested a perception of pain
after O3/O2-insufflation, despite reduced writhing actions during the
writhing behavior experiment.
From qPCR-data it is concluded that neither the H3C-COOHintraperitoneal-
injection nor the insufflation of O3/O2-gas-mixture provide
immunostimulative effects.
This means that a therapeutic application of O3/O2 gas mixture should only
be performed under adequate analgesia. A pathological immune
stimulation caused by intraperitoneal O3/O2 insufflation however, is not to
be expected.
Review
Metadata
Contributors
Supervisor:
Dates
Created: 2014Issued: 2014-02-19Updated: 2014-02-19
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
painintraperitoneal O3/O2-insufflation-therapyimmune system
DFG-subjects
ImmunsystemSchmerzOzontherapie
DDC-Numbers
610
show more
Springmann, Volker Christian (1051975530): Auswirkung von O3/O2-Gasgemisch im Peritoneum auf Zytokinproduktion und Schmerzinduktion. : Philipps-Universität Marburg 2014-02-19. DOI: https://doi.org/10.17192/z2014.0185.
License
This item has been published with the following license: In Copyright