Kationenpermeabilität von Erythrozyten bei niedriger Ionenstärke in Abhängigkeit bestimmter Anionen und Inhibitoren unter besonderer Berücksichtigung des Glucosetransporters
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Philipps-Universität Marburg
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We have reported in human red blood cells in addition to an increase of K+ and Na+ pereability a decrease in glucose efflux in low-chloride media. Pig red cells without glucose transporter do not show such cation efflux. A substantial portion of cation efflux in human red blood cells is inhibited by DIDS in low-anion concentration. We added DIDS one minute after Cl-/OH--exchange and measured in K+-efflux a Ki of 98 nM DIDS. It is without effect in regular glucose efflux, but reverses partly inhibition of glucose efflux in low-chloride media. Phenylglyoxal inhibits both cation efflux in low-chloride media. We found at 0.8 mM chloride by adding SDS after Cl-/OH--exchange an activation of cation efflux with an EC50 of 17
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This item has been published with the following license: In Copyright