Item type:Thesis, Open Access

Untersuchungen zum Kerntransport von Phosphoprotein pp65 in HCMV infizierten menschlichen Fibroblasten

Thumbnail Image

Files

dkbs.pdf (4.22 MB)

Date

2007-07-12

Authors

Publisher

Philipps-Universität Marburg
DOI

Supervisors

Abstract

Human cytomegalovirus (HCMV) open reading frame UL83 codes for the phosphoprotein pp65 (ppUL83), an abundant constituent of the virion tegument. It is also detectable in DBs and NIEPs. Immediately after infection pp65 is targeted to cell nucleus. This transport is mediated by two nuclear localization signals. New synthesized pp65 is detectable in the cell nucleus 4 hours after infection. Pulse chase experiments were used to show the nuclear transport of pp65 in the late phase of virion replikation. A statement about the quantity of this nuclear transport was not possible. Therefore transfectionexperiments were used to show the nuclear transport of pp65 in vivo. For this a fusionprotein of pp65 and the green fluorescent protein (GFP) was used, which was localized in the pcDNA3.1 vector. Using the confocal laser scanning microscopy, green fluorescent structures were detectable up to 12 hours after infection.

Review

Metadata

Contributors
Supervisor:
Dates
Created: 2007Issued: 2007-07-12Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2007.0373
Faculty
Medizin
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
Phosphoprotein pp65Nuclear transportPhosphoprotein pp65TegumentproteinTegumentproteinKerntransport
DFG-subjects
Cytomegalie-Virus
DDC-Numbers
610
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2007.0373
show more
Bringezu-Schröder, Kerstin: Untersuchungen zum Kerntransport von Phosphoprotein pp65 in HCMV infizierten menschlichen Fibroblasten. : Philipps-Universität Marburg 2007-07-12. DOI: https://doi.org/10.17192/z2007.0373.

License

item.page.license.under-copyright
This item has been published with the following license: In Copyright