Item type:Doctoral Thesis, Open Access

Nachweis der Humanbelastung und klinische Relevanz des Weichmachers DEHP, sowie ausgewählter Metabolite

Thumbnail Image

Files

dhj.pdf (2.94 MB)

Date

2006-12-21

Authors

Publisher

Philipps-Universität Marburg
DOI

Supervisors

Abstract

Aim of the work was the development and validation of a routine method for the detection of the plasticizers Di(ethylhexyl)phthalate (DEHP) together with its primary and secondary metabolites Mono(ethylhexyl)phthalate, ethylhexanol, phthalic acid and ethylhexanoic acid. The analysis was performed by gas-chromatography mass spectrometry after fluid-fluid extraction and derivatisation with MBDSTFA. After evaluation, the method was tested in a small pilot project comparing the exposure of plasticizers in patients undergoing haemodialysis and a control group. Almost all analysts were detected to be higher in haemodialysis patients. The only exception was Mono(ethylhexyl)phthalate, with a slightly lower amount Besides the analytical part, experiments on the immunotoxicological effect of DEHP were carried out on in vitro basis with exposure experiments on Jurkat E6.1 lymphocytes, as well as in vivo experiments investigating the effect in the lungs of DEHP exposed BALB/c mice after short-term and long-term exposure.

Review

Metadata

Contributors
Supervisor:
Dates
Created: 2006Issued: 2006-12-21Updated: 2011-08-10
DOI
https://doi.org/10.17192/z2006.0917
Faculty
Fachbereich Chemie
Publisher
Philipps-Universität Marburg
Language
ger
Data types
DoctoralThesis
Keywords
DEHPUrin,ExposurePlasticiserMausAnalyticDEHPMice
DFG-subjects
WeichmacherSerumReal time quantitative PCRInstrumentelle AnalytikBelastungTierversuchAnalyse
DDC-Numbers
540
License
https://rightsstatements.org/vocab/InC-NC/1.0/
URI
https://open.uni-marburg.de/handle/10.17192/z2006.0917
show more
Jungclas, Henrik (132434784): Nachweis der Humanbelastung und klinische Relevanz des Weichmachers DEHP, sowie ausgewählter Metabolite. : Philipps-Universität Marburg 2006-12-21. DOI: https://doi.org/10.17192/z2006.0917.

License

item.page.license.under-copyright
This item has been published with the following license: In Copyright