Protocol for analyzing T cell and macrophage motility in a mouse pancreatic cancer spheroid model using light-sheet microscopy

The interaction of macrophages and T cells is pivotal for shaping the immune response to cancer. Here, we present a protocol for investigating the co-culture of macrophages and T cells in a pancreatic cancer spheroid model. We describe steps for analyzing migration patterns by light-sheet microscopy, generating murine bone marrow-derived macrophages (BMDMs) and cytotoxic T lymphocytes (CTLs), and staining CTLs for image analysis. We then detail procedures for generating spheroids using a mouse pancreatic cancer cell line.

Metadata

Relations

Is version of: https://doi.org/10.1016/j.xpro.2025.103981

DOI

https://doi.org/10.17192/openumr/1016

Publisher

Elsevier

Language

en

Data types

JournalArticle

Keywords

Cell cultureImmunologyMicroscopy

Funding

Funding Organisations:

Philipps-Universität Marburg

Project Information:Gefördert durch den Open-Access-Publikationsfonds der UB Marburg.

License

Attribution 4.0 International

URI

https://open.uni-marburg.de/handle/openumr/11468

Vanessa Zimmer; Slusny, Benedikt; Roth, Katrin; Bauer, Christian: Protocol for analyzing T cell and macrophage motility in a mouse pancreatic cancer spheroid model using light-sheet microscopy. In: STAR Protocols. Volume 6, Issue 3, 19 September 2025, 103981, Jg. (), S. 1-18. DOI: https://doi.org/10.17192/openumr/1016.